VDAC1 Antibody (YA016)

VDAC1 Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to VDAC1. It can be used as a loading control antibody.

VDAC1 protein serves as a versatile channel, permeating both the mitochondrial outer membrane and the plasma membrane. This dual functionality is pivotal, as the channel in the outer mitochondrial membrane facilitates the diffusion of small hydrophilic molecules, while in the plasma membrane, it plays a role in cell volume regulation and apoptosis. VDAC1 exhibits a dynamic conformational switch, adopting an open state at low or zero membrane potential and a closed state at potentials above 30-40 mV. The open state displays weak anion selectivity, whereas the closed state exhibits cation selectivity. Notably, VDAC1 binds various signaling molecules, including the sphingolipid ceramide, phospholipid phosphatidylcholine, and sterol cholesterol. In depolarized mitochondria, VDAC1 acts downstream of PRKN and PINK1, participating in mitophagy promotion or apoptosis prevention. Polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx, ultimately inhibiting apoptosis. Additionally, VDAC1 may contribute to the formation of the permeability transition pore complex (PTPC), which releases mitochondrial products triggering apoptosis. Furthermore, it has been implicated in mediating ATP export from cells and is subject to inhibition by nitric oxide. The multifaceted functions of VDAC1 underscore its significance in various cellular processes, ranging from ion transport to cellular homeostasis and apoptosis regulation.

Free

P21796

Predicted band size: 30 kDa; Observed band size: 30 kDa

Protein A affinity purified.

Mitochondrion outer membrane, Cell membrane, Membrane raft

Non-conjugated

Unmodified

AB_3102459

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:10,000; ICC: 1:50-100; IHC-P: 1:200-1:1000; IF-Tissue: 1:200

• 
  Western blot analysis of extracts from MDA-MB231(lane 2(20μg), Hela(lane 3(20μg) and K562(lane 4(20μg) using VDAC1(HY-P80369) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling VDAC1 with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling VDAC1with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IF-Tissue

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within N-terminal human VDAC1.