USP28 Antibody (YA1336)

USP28 Antibody (YA1336) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1336), targeting USP28, with a predicted molecular weight of 122 kDa (observed band size: 135 kDa). USP28 Antibody (YA1336) can be used for WB, ICC/IF, FC experiment in human background.

The protein encoded by The related gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of The related gene is seen in several cancers.

Q96RU2

Predicted band size: 122 kDa; Observed band size: 135 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3103703

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IF: 1/50-1/200;FC: 1/50-1/100

• 
  Western blot analysis of extracts from A431 (lane 2(20μg) , HEK293T (lane 3(20μg) and Hela (lane 4(20μg) using USP28 (HY-P81591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, ICC/IF, FC

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human USP28