uPAR Antibody

uPAR Antibody is a rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to uPAR. It can be applicated for WB, IHC-P assays, in the background of human.

uPAR Protein serves as a receptor for urokinase plasminogen activator, playing a crucial role in localizing and facilitating plasmin formation. Additionally, it mediates the proteolysis-independent signal transduction activation effects of U-PA. Subject to negative-feedback regulation by U-PA, uPAR undergoes cleavage into an inactive form. It typically exists as a monomer, and interacts with various proteins, including SRPX2 and MRC2. Notably, it forms a complex with FAP (seprase) at the cell surface of invadopodia membrane, contributing to cellular processes involved in proteolysis and signaling. Moreover, uPAR engages in a ternary complex with PLAU (urokinase-type plasminogen activator) and SERPINE1, and this complex also interacts with SORL1, further highlighting its multifaceted interactions in cellular functions.

Q03405

Predicted band size: 37 kDa;Observed band size: 37 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3676573

Cardiovascular

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

WB: 1/500-1/1000; IHC: 1/50-1/100;

• 
  Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using uPAR Antibody (HY-P81512A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Fusion protein of human PLAUR.