Tyrosine Hydroxylase Antibody (YA023)

Tyrosine Hydroxylase Antibody (YA023) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tyrosine Hydroxylase.

Tyrosine hydroxylase is an enzyme that plays a pivotal role in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline. It catalyzes the rate-limiting step in this pathway, converting L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) through the use of tetrahydrobiopterin and molecular oxygen. While its primary substrate is tyrosine, tyrosine hydroxylase also exhibits lower specificity and can hydroxylate phenylalanine and tryptophan. This enzymatic activity is essential for the synthesis of neurotransmitters involved in various physiological processes. Moreover, tyrosine hydroxylase has been found to positively regulate the regression of retinal hyaloid vessels during postnatal development, indicating its diverse roles beyond catecholamine biosynthesis.

Free

P07101

Predicted band size: 59 kDa; Observed band size: 59 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102454

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:10,000 ;ICC: 1:50-1:200 ;IHC-P: 1:2,000 ;FC: 1:50-1:100 ;IF: 1:200

• 
  Immunocytochemistry analysis of C6 cells labeling Tyrosine Hydroxylase with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of C6 cells labeling Tyrosine Hydroxylase with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosine Hydroxylase Antibody (HY-P80362)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Tyrosine Hydroxylase Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80362, 1/2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Tyrosine Hydroxylase Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80362, 1/2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within C-terminal human Tyrosine Hydroxylase.