Transcription Factor EB Antibody (YA3266)

Transcription Factor EB Antibody (YA3266) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Factor EB.

TFEB protein operates as a key transcription factor orchestrating diverse cellular processes, including lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism, and immune response. Recognizing E-box sequences, TFEB's efficient DNA-binding necessitates dimerization with itself or other MiT/TFE family members like TFE3 or MITF. It responds to amino acid availability through MTOR-mediated phosphorylation, promoting cytosolic retention and inactivation. Conversely, MTOR inhibition during starvation or lysosomal stress induces TFEB dephosphorylation, leading to nuclear localization and transcriptional activation. TFEB specifically binds CLEAR-box sequences, activating the expression of lysosomal genes and regulating lysosomal positioning. Additionally, it positively regulates autophagy, contributes to T-cell-dependent antibody responses, and plays a role in vascularization of the placenta. In response to bacterial infection, TFEB mediates itaconate-induced alkylation, activating lysosomal biogenesis and aiding in bacterial clearance. Notably, the eltrombopag drug inhibits TFEB by binding to the bHLH domain and disrupting DNA-binding.

P19484

Predicted band size: 53 kDa; Observed band size: 65-70 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105631

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis of extracts from A549 (lane 2(20μg), A549 (lane 3(40μg), using Transcription Factor EB Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human TFEB