TRAF2 Antibody (YA032)

TRAF2 Antibody (YA032) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAF2.

TRAF2 is an E3 ubiquitin-protein ligase that plays a central role in regulating NF-kappa-B and JNK activation, thereby influencing cell survival and apoptosis. It catalyzes 'Lys-63'-linked ubiquitination of target proteins such as BIRC3, IKBKE, MLST8, RIPK1, and TICAM1, and serves as an essential component of multiple E3 ubiquitin-protein ligase complexes, facilitating ubiquitination by bridging target proteins with other E3 ligases. TRAF2 regulates the protein levels of BIRC2 and BIRC3 by inhibiting their autoubiquitination and subsequent degradation, a process independent of its RING-type zinc finger domain. Additionally, TRAF2 mediates NF-kappa-B activation by EIF2AK2/PKR and, in complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. In the mTOR pathway, TRAF2 modulates the assembly of mTORC1 and mTORC2 by mediating 'Lys-63'-linked ubiquitination of MLST8, inhibiting mTORC2 formation while promoting mTORC1 assembly. TRAF2 is also required for normal antibody isotype switching from IgM to IgG (by similar).

Free

Q12933

Predicted band size: 56 kDa; Observed band size: 53 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102451

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:5,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) or lane 3(40μg)) and K562 (lane 4(20μg) or lane 5(40μg)) using TRAF2 (HY-P80357) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using TRAF2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80357, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using TRAF2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80357, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within N-terminal human TRAF2.