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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Topoisomerase I Antibody (YA2237)

Topoisomerase I Antibody (YA2237)

Cat. No.: HY-P82492
COA
SDS
User Guide for Antibodies Technical Support

Topoisomerase I Antibody (YA2237) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2237), targeting Topoisomerase I, with a predicted molecular weight of 91 kDa (observed band size: 91 kDa). Topoisomerase I Antibody (YA2237) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Topoisomerase I Antibody (YA2237) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2237), targeting Topoisomerase I, with a predicted molecular weight of 91 kDa (observed band size: 91 kDa). Topoisomerase I Antibody (YA2237) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, mouse, rat background.
Background

TOP1 protein plays a pivotal role in cellular processes by alleviating the supercoiling and torsional tension of DNA generated during DNA replication and transcription. This is achieved through the transient cleavage and subsequent rejoining of one strand of the DNA duplex. The enzyme introduces a single-strand break via transesterification at a specific target site in duplex DNA, leading to the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate. This intermediate expels a 5'-OH DNA strand, allowing the free DNA strand to rotate around the intact phosphodiester bond on the opposing strand, effectively removing DNA supercoils. In the final religation step, the DNA 5'-OH attacks the covalent intermediate, leading to the expulsion of the active-site tyrosine and restoration of the DNA phosphodiester backbone. Beyond its role in DNA topology maintenance, TOP1 is involved in the regulation of alternative splicing, exemplified by its impact on tissue factor (F3) pre-mRNA in endothelial cells. Additionally, it contributes to the circadian transcription of the core circadian clock component BMAL1 by modifying the chromatin structure surrounding the ROR response elements (ROREs) on the BMAL1 promoter.
Gene ID
SwissProt ID
Synonyms
TOP1; DNA topoisomerase 1; DNA topoisomerase I
Molecular Weight

Predicted band size: 91 kDa; Observed band size: 91 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200
Application

WB, IHC-F, IHC-P, ICC/IF
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human Topoisomerase I
Database

Entrez Gene: 7150 Human ; 21969 Mouse ; 64550 Rat

SwissProt: P11387 Human ; Q04750 Mouse ; Q9WUL0 Rat

OMIM: 126420 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Topoisomerase I Antibody (YA2237) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Topoisomerase I Antibody (YA2237)
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