Thrombospondin 1 Antibody (YA3553)

Cat. No.: HY-P83750: Data Sheet COA
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Thrombospondin 1 Antibody (YA3553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombospondin 1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Publications Citing Use of MCE Thrombospondin 1 Antibody (YA3553)

•J Orthop Surg Res. 2025 May 20;20(1):488. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Thrombospondin 1 Antibody (YA3553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombospondin 1.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 129 kDa;

Observed band size: 170 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07996

Gene ID

7057 [NCBI]

Immunogen

Synthetic peptide within human Thrombospondin 1 aa 500-1,170 / 1,170.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000-1:8000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:500
FC FC: Flow Cytometry	1:1000

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HUVEC (lane 2(40μg) and HUVEC (lane 3(60μg) using Thrombospondin 1(HY-P83750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Synonyms

TSP; THBS; TSP1; TSP-1; THBS-1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)