TERT Antibody (YA3439)

TERT Antibody is an unconjugated, approximately 125 kDa, rabbit-derived, anti-TERT monoclonal antibody. TERT Antibody can be used for: WB, IHC-P, Flow-Cyt expriments in human background without labeling.

TERT is the catalytic subunit of telomerase, a ribonucleoprotein enzyme critical for chromosome end replication in most eukaryotes. It is active in progenitor and cancer cells but exhibits minimal or no activity in normal somatic cells. As the core component of the telomerase holoenzyme complex, TERT elongates telomeres by functioning as a reverse transcriptase that adds TTAGGG repeats to chromosomal termini using an internal RNA template. Its catalytic cycle involves primer binding, extension, and product release upon reaching template boundaries. TERT shows higher activity on substrates containing 2-3 telomeric repeats, with regulation mediated by associated proteins, chaperones, and post-translational modifications. It also modulates Wnt signaling and plays pivotal roles in aging and antiapoptosis processes.

O14746

Predicted band size: 127 kDa; Observed band size: 127 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human

WB: 1/500-1/1000; IHC: 1/50-1/100; FC: 1/50-1/100

• 
  Western blot analysis of extracts from K562 using TERT antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, FC

Liquid

Supplied in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human TERT.