TATA Box Binding Protein Antibody (YA3862)

TATA Box Binding Protein Antibody (YA3862) is a mouse-derived and non-conjugated IgG1 antibody, targeting to TBP. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

TATA Box Binding Protein (TBP) is a core component of the TFIID basal transcription factor complex, playing a pivotal role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TBP recognizes and binds promoters with or without a TATA box, facilitating the assembly of the pre-initiation complex (PIC). The TFIID complex comprises TBP and TBP-associated factors (TAFs), including TAF1 to TAF13, and is structurally divided into three modules: TFIID-A, TFIID-B, and TFIID-C, with TBP forming the TFIID-A module alongside TAF3 and TAF5. During PIC assembly, TBP binds and bends promoter DNA. Additionally, TBP is part of a BRF2-containing transcription factor complex that regulates RNA polymerase III-mediated transcription, and it is a component of the SL1/TIF-IB transcription factor complex involved in RNA polymerase I-dependent PIC assembly. SL1 also stabilizes the binding of nucleolar transcription factor 1/UBTF to rDNA.

P20226

Predicted band size: 38 kDa; Observed band size: 35-45 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human TBP (AA: 1-144) expressed in E. Coli.