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  5. TATA Box Binding Protein Antibody (YA3260)

TATA Box Binding Protein Antibody (YA3260)

Cat. No.: HY-P83515
COA
SDS
User Guide for Antibodies Technical Support

TATA Box Binding Protein Antibody (YA3260) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3260), targeting TATA Box Binding Protein, with a predicted molecular weight of 38 kDa (observed band size: 38 kDa). TATA Box Binding Protein Antibody (YA3260) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

TATA Box Binding Protein Antibody (YA3260) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3260), targeting TATA Box Binding Protein, with a predicted molecular weight of 38 kDa (observed band size: 38 kDa). TATA Box Binding Protein Antibody (YA3260) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse background.
Background

TATA Box Binding Protein (TBP) is a core component of the TFIID basal transcription factor complex, playing a pivotal role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TBP recognizes and binds promoters with or without a TATA box, facilitating the assembly of the pre-initiation complex (PIC). The TFIID complex comprises TBP and TBP-associated factors (TAFs), including TAF1 to TAF13, and is structurally divided into three modules: TFIID-A, TFIID-B, and TFIID-C, with TBP forming the TFIID-A module alongside TAF3 and TAF5. During PIC assembly, TBP binds and bends promoter DNA. Additionally, TBP is part of a BRF2-containing transcription factor complex that regulates RNA polymerase III-mediated transcription, and it is a component of the SL1/TIF-IB transcription factor complex involved in RNA polymerase I-dependent PIC assembly. SL1 also stabilizes the binding of nucleolar transcription factor 1/UBTF to rDNA.
Gene ID
SwissProt ID
Synonyms
TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit
Molecular Weight

Predicted band size: 38 kDa; Observed band size: 38 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20
Application

WB, IHC-F, IHC-P, ICC/IF, IP
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human TATA binding protein TBP
Database

Entrez Gene: 6908 Human ; 21374 Mouse ;

SwissProt: P20226 Human ; P29037 Mouse ;

OMIM: 607136 Human

Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TATA Box Binding Protein Antibody (YA3260) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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