STAT5B Antibody (YA052)

STAT5B Antibody (YA052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5B.

STAT5B, a multifunctional protein, orchestrates signal transduction and transcriptional activation, playing a pivotal role in cellular responses to various stimuli, including the cytokine KITLG/SCF and other growth factors. Functioning as a transcription factor, STAT5B binds to the GAS element and facilitates PRL-induced transcription, positively regulating hematopoietic/erythroid differentiation. Upon activation, it forms homodimers and heterodimers with related family members, contributing to its diverse functional roles. Additionally, STAT5B engages in protein-protein interactions, including binding to NR3C1 and forming complexes with NCOA1, NMI, and SOCS7. Notably, its interaction with INSR, facilitated by the SH2 domain, highlights its involvement in insulin signaling. Moreover, the interaction with CPEB3 serves to inhibit STAT5B-mediated transcriptional activation, adding a layer of regulatory complexity to its diverse cellular functions.

Free

P51692

Predicted band size: 90 kDa; Observed band size: 90 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102445

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:2,000; ICC/IF: 1:100-1:500; IHC-P: 1:50-1:200; IF-Tissue: 1:50-1:200; IP: 1-2 μg/sample; FC: 1:1,000

• 
  Western blot analysis of extracts from K562(lane 2(20μg), Hela(lane 3(20μg), Jurkat(lane 4(20μg) ) using STAT5b (HY-P80346) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80346, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, IP, FC, IF-Tissue

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within C-terminal mouse STAT5b.