STAT1 Antibody (YA059)

STAT1 Antibody (YA059) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT1.

STAT1 is a signal transducer and transcription activator that plays a crucial role in mediating cellular responses to interferons (IFNs), cytokine KITLG/SCF, and various growth factors. Upon binding of type I IFN (IFN-alpha and IFN-beta) to cell surface receptors, STAT1 undergoes tyrosine phosphorylation and forms a dimer with STAT2. This complex, known as ISGF3 transcription factor, translocates to the nucleus and activates the transcription of IFN-stimulated genes (ISG), establishing an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is phosphorylated at both tyrosine and serine residues, forming a homodimer (GAF) that enters the nucleus and binds to the IFN-gamma activated sequence (GAS), driving the expression of target genes and inducing a cellular antiviral state. Additionally, STAT1 becomes activated in response to KITLG/SCF and KIT signaling and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3, and FGFR4. Furthermore, in the small intestine, STAT1 associates with Gasdermin-D and promotes the transcription of CIITA, inducing the formation of type 1 regulatory T (Tr1) cells. STAT1 interacts with various proteins, including PIAS1, IFNAR1, IFNAR2, NMI, CREBBP/CBP, PTK2/FAK1, SRC, ERBB4, PARP9, DTX3L, EP300/p300, and IFNGR1, contributing to its diverse cellular functions.

Free

P42224

Predicted band size: 87 kDa; Observed band size: 87 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102439

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:2000; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg), A549 (lane 4(20μg) and HEK293(lane 5(20μg) using STAT1(HY-P80340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 RAW264.7 cells labeling STAT1 Antibody (HY-P80340, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human STAT1.AA range:710-750.