SP1 Antibody (YA6312)

SP1 Antibody (YA6312) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SP1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human.

SP1 is a transcription factor capable of activating or repressing transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in processes such as cell growth, apoptosis, differentiation, and immune responses. SP1 is highly regulated by post-translational modifications (e.g., phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation). It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage while participating in chromatin remodeling. SP1 plays an essential role in regulating FE65 gene expression and, in complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 exhibits stronger transcriptional activation than isoform 1 and positively regulates the core clock component BMAL1. Additionally, SP1 facilitates the recruitment of SMARCA4/BRG1 to the c-FOS promoter. By similarity, SP1 protects cells against oxidative stress following brain injury by regulating RNF112 expression.

P08047

Predicted band size: 100 kDa; Observed band size: 100 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human

IHC: 1:200-1000; WB: 1:1000-5000; ICC/IF: 1:200-1000; ELISA: 1:5000-20000; IP: 1:50-200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG