SOX2 Antibody (YA4045)

SOX2 Antibody (YA4045) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOX2.

Sox2, a pivotal transcription factor, forms a trimeric complex with OCT4 on DNA, exerting control over the expression of genes crucial in embryonic development, such as YES1, FGF4, UTF1, and ZFP206. It binds to the proximal enhancer region of NANOG, playing a critical role in early embryogenesis and maintaining embryonic stem cell pluripotency. Functioning as a downstream target of SRRT, Sox2 contributes to the promotion of neural stem cell self-renewal while preventing neural cell differentiation through counteraction against proneural proteins. Additionally, it may serve as a switch in neuronal development. Sox2 engages in various interactions, including those with ZSCAN10, SOX3, FGFR1, GLIS1, POU5F1, DDX56, L3MBTL3, DCAF5, RCOR1/CoREST, PHF20L1, and TRIM26, underscoring its dynamic regulatory network. These interactions highlight its involvement in critical cellular processes, including ubiquitination, degradation protection, and prevention of ubiquitination by specific ligases.

P48431

Predicted band size: 34 kDa; Observed band size: 34 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit, Monkey

WB: 1:500-1:1000; IHC: 1:200-1:1000; IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling SOX2 with SOX2 Antibody (HY-P84348)at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX2 Antibody (HY-P84348) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling SOX2 with SOX2 Antibody (HY-P84348) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX2 Antibody (HY-P84348) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using SOX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (30144, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using SOX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (30144, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SOX2 aa 1-170.