SNAI1 Antibody

SNAI1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SNAI1.

SNAI1 is a protein involved in the induction of epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, cell survival, and migration. It binds to three E-boxes in the E-cadherin/CDH1 gene promoter and the promoters of CLDN7 and KRT8, recruiting the histone demethylase KDM1A to these promoters, leading to decreased dimethylated H3K4 levels and transcriptional repression. The N-terminal SNAG domain competes with histone H3 for binding to the histone demethylase complex formed by KDM1A and RCOR1, thereby inhibiting demethylation of histone H3 at Lys-4 (in vitro). During EMT, SNAI1 cooperates with LOXL2 to negatively regulate pericentromeric heterochromatin transcription (By similar: SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription, resulting in the release of the heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits). Additionally, SNAI1 associates with EGR1 and SP1 to mediate TPA-induced upregulation of CDKN2B, potentially by binding to the 5'-TCACA-3' sequence in the CDKN2B promoter region, and may also activate the CDKN2B promoter independently.

O95863

Predicted band size: 29 kDa; Observed band size: 29 kDa

affinity purified

Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Non-conjugated

Unmodified

AB_3103067

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; ELISA: 1:10000

• 
  Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using SNAI1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using SNAI1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using SNAI1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

The antiserum was produced against synthesized peptide derived from human SNAI1.