SIRT1 Antibody (YA4425)

SIRT1 Antibody (YA4425) is a mouse-derived and non-conjugated IgG1 antibody, targeting to SIRT1. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, rabbit, monkey.

SIRT1, a NAD-dependent protein deacetylase, orchestrates the integration of various cellular functions, including cell cycle progression, response to DNA damage, metabolism, apoptosis, and autophagy, by modulating the acetylation status of numerous target proteins. Functioning as a sensor of the NAD(+)/NADH ratio, SIRT1 responds to changes in cellular energetics induced by factors such as glucose deprivation and caloric restriction. Its wide-ranging impact encompasses chromatin remodeling through histone deacetylation, ultimately leading to transcriptional regulation. SIRT1 engages in diverse interactions, deacetylating transcription factors, coregulators, and histones, thereby exerting both positive and negative regulation on target gene expression. Additionally, SIRT1 is implicated in critical processes such as DNA damage response, autophagy, circadian rhythm regulation, and metabolic homeostasis. Its multifaceted roles extend to the regulation of various cellular pathways and the maintenance of genomic integrity, highlighting its significance in cellular physiology and stress response.

Q96EB6

Predicted band size: 82 kDa; Observed band size: 110 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SIRT1 expressed in E. Coli.