RICTOR Antibody (YA4339)

RICTOR Antibody (YA4339) is a mouse-derived and non-conjugated IgG1 antibody, targeting to RICTOR. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, monkey.

RICTOR is a core component of the mechanistic target of rapamycin complex 2 (mTORC2), which relays growth factor signals to pathways governing proliferation, cytoskeletal organization, lipogenesis, and anabolic metabolism. mTORC2 phosphorylates and activates AGC kinase family members (e.g., AKT, PKC, and SGK1) and, unlike mTORC1, is insensitive to nutrient availability. RICTOR likely functions as a molecular scaffold within mTORC2 and confers FKBP12-rapamycin insensitivity. The complex mediates context-dependent phosphorylation of AKT1 (e.g., at Thr-450, Ser-473) for full activation, and phosphorylates SGK1 (Ser-422) and PRKCA (Ser-657) (by similar). It also regulates insulin signaling (e.g., via FBXW8 and IGF2BP1 phosphorylation) (by similar) and modulates actin cytoskeleton dynamics through Rho GTPases.

Q6R327

Predicted band size: 192 kDa; Observed band size: 192 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), HepG2 (lane 3(20μg) and HEK293(lane 4(20μg) using RICTOR (HY-P84642) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling RICTOR with RICTOR antibody (30090) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RICTOR antibody (30090) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling RICTOR with RICTOR antibody (30090) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RICTOR antibody (30090) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human RICTOR expressed in E. Coli.