RhoA Antibody (YA093)

RhoA Antibody (YA093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RhoA.

RhoA Protein is a small GTPase crucial for orchestrating diverse cellular responses, cycling between an active GTP-bound state and an inactive GDP-bound state. Primarily associated with the organization of the cytoskeleton, the active form of RhoA binds to various effector proteins, influencing cellular processes such as cytoskeletal dynamics, cell migration, and cell cycle progression. It plays a pivotal role in regulating signal transduction pathways that connect plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Additionally, RhoA is indispensable for microtubule-dependent signals required for myosin contractile ring formation during cell cycle cytokinesis, contributing to cleavage furrow formation. Essential for apical junction formation in keratinocyte cell-cell adhesion, RhoA also participates in the MEMO1-RHOA-DIAPH1 signaling pathway, influencing ERBB2-dependent microtubule stabilization. It further modulates potassium channel activity, regulates guanine nucleotide exchange factors, and acts as a target for the yopT cysteine peptidase from Yersinia pestis during microbial infection.

Free

P61586

Predicted band size: 22 kDa; Observed band size: 22 kDa

Protein A affinity purified.

Cell membrane, Cytoplasm, Cleavage furrow, Midbody, Cell projection.

Non-conjugated

Unmodified

AB_3102419

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:2000; ICC/IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , THP-1 (lane 3(20μg) , SiHa (lane 4(20μg) , C2C12 (lane 4(20μg),using RhoA Antibody (HY-P80311). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 Jurkat cells labeling RhoA Antibody(HY-P80311, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/150 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, ICC/IF, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human.AA range:144-193.