RFA2 Antibody (YA5283)

RFA2 Antibody (YA5283) is a mouse-derived and non-conjugated monoclonal antibody, targeting to RFA2. It can be applicated for WB, IP, ICC assays, in the background of human.

RFA2 is a component of the heterotrimeric replication protein A complex (RPA/RP-A) that binds and stabilizes single-stranded DNA intermediates formed during DNA replication or stress. It prevents their reannealing while recruiting and activating proteins and complexes involved in DNA metabolism, playing essential roles in DNA replication and cellular DNA damage response. Within the damage response, the RPA complex regulates DNA repair and checkpoint activation by recruiting ATRIP to activate the master regulator ATR kinase. It facilitates chromatin recruitment of double-strand break repair factors RAD51 and RAD52, as well as nucleotide excision repair proteins like XPA and XPG to damage sites. RFA2 also likely participates in base excision repair (BER) via interaction with UNG and recruits SMARCAL1/HARP for replication fork restart. Additionally, it may contribute to telomere maintenance and stimulates the 5'-3' helicase activity of BRIP1/FANCJ.

P15927

Observed band size: 32 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:2000; ICC: 1:200

WB, IP, ICC

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.