Rad51 Antibody (YA108)

Rad51 Antibody (YA108) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad51.

Rad51 plays a crucial role in homologous strand exchange, a key step in DNA repair via homologous recombination (HR). It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for homology search and strand exchange. Rad51 catalyzes the recognition of homology and strand exchange between homologous DNA partners, forming a joint molecule between broken DNA and the repair template. During replication stress, Rad51 is recruited to resolve stalled replication forks. Additionally, Rad51 is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to function in DNA repair through HR. Under oxidative stress conditions, Rad51, along with RAD51C and XRCC3, regulates mitochondrial DNA copy number. Rad51 is also involved in interstrand cross-link repair.

Free

Q06609

Predicted band size: 37 kDa; Observed band size: 37 kDa

Protein A affinity purified.

Chromosome, Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus.

Non-conjugated

Unmodified

AB_3102412

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000 ;ICC/IF: 1:50-1:100 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Jurkat (lane 2(20μg)，NIH3T3 (lane 3(20μg) and HEK293(lane 4(20μg) using Rad51 (HY-P80297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Rad51 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80297, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Rad51 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80297, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Rad51.AA range:1-50.