RAB8A Antibody (YA109)

RAB8A Antibody (YA109) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAB8A.

RAB8A is a small GTPase belonging to the Rab family, serving as a key regulator of intracellular membrane trafficking. It cycles between GDP-bound (inactive) and GTP-bound (active) states to recruit downstream effector proteins, regulating vesicle formation, movement, tethering, and fusion. RAB8A is involved in polarized vesicular trafficking, neurotransmitter release, and collaborates with RAB11A, RAB3IP, the exocyst complex, and others to promote transcytosis of PODXL to apical membrane initiation sites (AMIS), apical surface formation, and lumenogenesis. It also regulates Golgi compact morphology, participates in epithelial cell polarization with MYO5B and RAB11A, and mediates membrane trafficking to the cilium and ciliogenesis. RAB8A may regulate adherens junction assembly via MICALL2 (by similarity) and play a role in insulin-induced GLUT4 translocation to the plasma membrane for glucose homeostasis (by similarity). Additionally, it is involved in autophagy, facilitates the export of newly synthesized proteins through a Rab8-Rab10-Rab11-dependent endosomal pathway, and is targeted to stressed lysosomes via LRRK2 phosphorylation, suppressing lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins.

Free

P61006

Predicted band size: 24 kDa; Observed band size: 24 kDa

Protein A affinity purified.

Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody.

Non-conjugated

Unmodified

AB_3102411

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) ，HepG2(lane 3(20μg) ,RAW264.7(lane 4(20μg) using RAB8A(HY-P80296) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human RAB8A.AA range:151-200.