PTGS2 Antibody (YA3971)

PTGS2 Antibody (YA3971) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PTGS2. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.

COX2 is a bifunctional enzyme with both cyclooxygenase and peroxidase activities, involved in the biosynthesis pathway of prostanoids, primarily derived from arachidonate (AA) and playing a key role in inflammatory responses. Its cyclooxygenase activity oxygenates AA to prostaglandin G2 (PGG2), which is then reduced by peroxidase activity to prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. Similarly, COX2 catalyzes the conversion of dihomo-gamma-linoleate (DGLA) and eicosapentaenoate (EPA) to PGH1 and PGH3, precursors of 1-series and 3-series prostaglandins, respectively. Additionally, COX2 metabolizes 2-arachidonoyl lysophospholipids and 2-arachidonoyl glycerol, contributing to prostanoid lysophospholipid production and pain response. The enzyme also generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism and produces resolvins during inflammation resolution, such as 17R-HDHA (a precursor of D-series resolvins) from docosahexaenoate (DHA) and 18S-HEPE (a precursor of E-series resolvins) from eicosapentaenoate (EPA). In vascular endothelial cells, COX2 converts docosapentaenoate (DPA) to 13R-HDPA, a precursor of 13-series resolvins (RvTs) that activate macrophage phagocytosis. In activated leukocytes, it oxygenates hydroxyeicosatetraenoates (HETEs) to diHETEs. COX2 can also utilize linoleate (LA) as a substrate to produce (9R)-HODE and (13S)-HODE in a regio- and stereospecific manner (By similarity). During neuroinflammation, COX2 is involved in neuronal secretion of 15R-lipoxin A4, which regulates phagocytic microglia (By similarity).

P35354

Predicted band size: 69 kDa; Observed band size: 74 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human PTGS2 (AA: 18-207) expressed in E. Coli.