PKC gamma Antibody (YA125)

PKC gamma Antibody (YA125) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKC gamma.

PKC gamma is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues. It regulates neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1 by increasing plasma membrane-associated GRIA4 expression, modulates sensitivity to opiates, pain, and alcohol, mediates synaptic function and cell survival after ischemia, and inhibits gap junction activity following oxidative stress. In primary cerebellar neurons, PKC gamma acts downstream of metabotropic glutamate receptor GRM5/MGLUR5, phosphorylating GRIN1/NMDAR1, which is critical for synaptic plasticity, synaptogenesis, excitotoxicity, and learning. While potentially involved in hippocampal long-term potentiation (LTP), it may not be essential for synaptic plasticity. PKC gamma contributes to desensitization of μ-opioid receptor-mediated G-protein activation in the spinal cord and is crucial for morphine-induced reinforcing effects in the limbic forebrain. It modulates μ-opioid receptor functionality via a signaling pathway leading to receptor phosphorylation and degradation, and may influence chronic morphine-induced nociceptive changes. The kinase also plays roles in neuropathic pain and maintains allodynia from peripheral inflammation. It mediates ethanol's behavioral effects and impacts on GABA(A) receptors, affecting initial sensitivity and tolerance. During cerebral ischemia, PKC gamma regulates neurotransmission and cell survival in synaptic membranes, participating in insulin-induced necrosis inhibition to minimize ischemic injury. It is required for eliminating multiple climbing fibers during cerebellar Purkinje cell innervation. In lens epithelial cells, hydrogen peroxide activates PKC gamma, which phosphorylates connexin-43 (GJA1/CX43), disassembling gap junction plaques and inhibiting activity—potentially protecting against oxidative stress (by similar). PKC gamma phosphorylates p53/TP53, promoting DNA damage-induced apoptosis, and resets the cerebral cortex circadian clock during restricted feeding by stabilizing BMAL1 via interference with ubiquitination (by similar). Additionally, it phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking.

Free

P05129

Predicted band size: 78 kDa; Observed band size: 78 kDa

Protein A affinity purified.

Cell membrane. Cytoplasm.

Non-conjugated

Unmodified

AB_3102517

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000; IF: 1/50-1/200

• 
  Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PKC gamma Antibody (HY-P80435) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human PKC gamma.