PIK3C3 Antibody (YA2789)

PIK3C3 Antibody (YA2789) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2789), targeting PIK3C3, with a predicted molecular weight of 102 kDa (observed band size: 102 kDa). PIK3C3 Antibody (YA2789) can be used for WB, IHC-P experiment in human, mouse, rat background.

PI3 Kinase Class 3 is the catalytic subunit of the PI3K complex, mediating the formation of phosphatidylinositol 3-phosphate. Different complex forms are involved in multiple membrane trafficking pathways: PI3KC3-C1 participates in the initiation of autophagosomes, while PI3KC3-C2 is involved in autophagosome maturation and endocytosis. As part of PI3KC3-C1, it promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. It also regulates degradative endocytic trafficking and is required for the abscission step in cytokinesis, likely in the context of PI3KC3-C2. By similarity, it is involved in the transport of lysosomal enzyme precursors to lysosomes and facilitates transport from early to late endosomes. In microbial infection, its kinase activity is essential for SARS-CoV-2 replication.

Q8NEB9

Predicted band size: 102 kDa; Observed band size: 102 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3105160

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100

• 
  Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using PIK3C3 Antibody (HY-P83044) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human PI 3 Kinase Class 3