Phospho-Tau (Ser396) Antibody (YA140)

Phospho-Tau (Ser396) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 79 kDa, targeting to Phospho-Tau(S396). It can be used for WB,ICC/IF,IHC-P,IHC-F,IF-Tissue assays with tag free, in the background of Mouse, Rat, Human.

Free

P10636

Predicted band size: 79 kDa;Observed band size: 70/130 kDa

Protein A affinity purified.

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Secreted.

Non-conjugated

Phosphorylated

AB_3102553

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Monkey, Pig

WB: 1:500-1:1,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:200;IHC-F:1:200-1:500; IF-Tissue:1:100-1:500

• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IHC-F, ICC/IF, IF-Tissue

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser396 of Human Tau.The exact sequence is proprietary to MCE.