Phospho-STAT3 (Ser727) Antibody (YA147)

Phospho-STAT3 (Ser727) Antibody (YA147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT3 (Ser727).

STAT3, a signal transducer and transcription activator, orchestrates cellular responses to various growth factors, interleukins, and cytokines. Upon activation, STAT3 recruits coactivators to the promoter region of target genes, thereby mediating diverse cellular processes such as proliferation, differentiation, and immune response regulation. It responds to signaling from interleukin-6, KITLG/SCF, and other growth factors, facilitating a cascade of downstream events. Additionally, STAT3 plays a role in cell cycle regulation, inflammatory response modulation, and energy homeostasis. Its functions extend to influencing apoptosis, melanocortin production, and insulin secretion. The protein's activation involves homodimerization or heterodimerization with related family members. STAT3 interacts with an array of molecules, including IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, and others, contributing to its intricate involvement in cellular signaling pathways.

Free

P40763

Predicted band size: 88 kDa; Observed band size: 88 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3102402

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat, Mouse

WB: 1:500-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser727 of Human STAT3.AA range:701-750.