Phospho-Rb (Ser807) Antibody (YA159)

Phospho-Rb (Ser807) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 106 kDa, targeting to Phospho-Rb(S807). It can be used for WB,ICC/IF,IHC-P assays with tag free, in the background of Human, Mouse.

Free

P06400

Predicted band size: 106 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Phosphorylated

AB_3102549

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000 ;ICC/IF: 1:50 ;IHC-P: 1:50-1:1,000

• 
  Immunocytochemistry analysis of L929 cells labeling Phospho-Rb (Ser807) with Phospho-Rb (Ser807) antibody (HY-P80476) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Rb (Ser807) antibody (HY-P80476) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg)using Phospho-Rb (HY-P80476) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80476, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of MCF-7 cells labeling Phospho-Rb (Ser807) with Phospho-Rb (Ser807) antibody (HY-P80476) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Rb (Ser807) antibody (HY-P80476) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser807 of Human Rb.The exact sequence is proprietary to MCE.