Phospho-PTEN (Ser380) Antibody (YA160)

Phospho-PTEN (Ser380) Antibody (YA160) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PTEN (Ser380).

PTEN is a dual-specificity protein phosphatase that dephosphorylates tyrosine-, serine-, and threonine-phosphorylated proteins. It also functions as a lipid phosphatase, preferentially acting on PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate to remove the phosphate group at the D3 position of the inositol ring. Additionally, PTEN acts as a cytosolic inositol 3-phosphatase, targeting Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate. By dephosphorylating phosphoinositides, PTEN antagonizes the PI3K-AKT/PKB signaling pathway, modulating cell cycle progression and survival. In motile cells, it suppresses lateral pseudopod formation, promoting polarization and directed movement. PTEN also dephosphorylates tyrosine-phosphorylated focal adhesion kinase, inhibiting migration, integrin-mediated spreading, and focal adhesion formation. It plays a key role in growth factor-induced epithelial cell migration, interacting with DLC1 or p85 to regulate RHOA and RAC1 activation. Furthermore, PTEN is a critical modulator of the AKT-mTOR pathway, influencing neuronal integration during adult neurogenesis (by similar). In excitatory hippocampal synapses, PTEN regulates synaptic function and plasticity, with its lipid phosphatase activity driving AMPA receptor-mediated synaptic depression, essential for NMDA receptor-dependent long-term depression (LTD) (by similar). PTEN may negatively regulate insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form exhibits stronger pro-apoptotic potential, while the cytoplasmic nonubiquitinated form has weaker tumor-suppressive activity.

Free

P60484

Predicted band size: 47 kDa; Observed band size: 52 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Secreted.

Non-conjugated

Phosphorylated

AB_3102116

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:2,000 ;ICC: 1:100-1:500

• 
  Immunocytochemistry analysis of MCF-7 cells labeling Phospho-PTEN (Ser380) Antibody (HY-P80475) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PTEN (Ser380) Antibody (HY-P80475) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-PTEN (Ser380) Antibody (HY-P80475) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PTEN (Ser380) Antibody (HY-P80475) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser380 of Human PTEN.The exact sequence is proprietary to MCE.