Phospho-PLK1 (Thr210) Antibody (YA161)

Phospho-PLK1 (Thr210) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 68 kDa, targeting to Phospho-PLK1(T210). It can be used for WB,ICC/IF,FC assays with tag free, in the background of Human.

Free

P53350

Predicted band size: 68 kDa

Protein A affinity purified.

Nucleus, Chromosome, Cytoplasm, Midbody.

Non-conjugated

Phosphorylated

AB_3102548

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000 ;ICC/IF: 1:10-1:50 ;FC: 1:10-1:50

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg), Calyculin A treated (HY-18983, 100ng/mL, 2hrs) as indicated using Phospho-PLK1 (Thr210)(HY-P80474) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of RAW264.7 cells labeling Phospho-PLK1 (Thr210) with Phospho-PLK1 (Thr210) antibody (HY-P80474) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PLK1 (Thr210) antibody (HY-P80474) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr210 of Human PLK1.AA range:181-230.