Phospho-PKC alpha (T638) Antibody (YA163)

Phospho-PKC alpha (T638) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 77 kDa, targeting to Phospho-PKC alpha (T638). It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.

Free

P17252

Predicted band size: 77 kDa

Protein A affinity purified.

Cytoplasm, Cell membrane, Mitochondrion membrane, Nucleus.

Non-conjugated

Phosphorylated

AB_3102400

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:5,000 ;ICC/IF: 1:50-1:500 ;IHC-P: 1:50-1:500 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from NIH3T3 (lane 2(20μg)，C6 (lane 3(20μg), Hela (lane 4(20μg) and HEK293(lane 5(20μg) using Phospho-PKC alpha (T638) (HY-P80279) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-PKC alpha (T638) with Phospho-PKC alpha (T638) Antibody (HY-P80279)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PKC alpha (T638) Antibody (HY-P80279) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr638 of Human PKC alpha.AA range:622-667.