Phospho-Met Antibody (YA175)

Cat. No.: HY-P80512: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-Met Antibody (YA175) is a non-conjugated and Rabbit origined monoclonal antibody about 156 kDa, targeting to Phospho-Met (pY1349). It can be used for WB,IHC-P assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-Met Antibody (YA175) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	Phospho-Met Antibody (YA175) is a non-conjugated and Rabbit origined monoclonal antibody about 156 kDa, targeting to Phospho-Met (pY1349). It can be used for WB,IHC-P assays with tag free, in the background of Human.
Tag	Free
Gene ID	4233 [NCBI]
SwissProt ID	P08581
Molecular Weight	Predicted band size: 156 kDa; Observed band size: 170/140 kDa
Purity	Protein A affinity purified.
Subcellular Location	Membrane, secreted.
Conjugation	Non-conjugated
Modification	Phosphorylated
RRID	AB_3102585
Research Field	Signal Transduction
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human
Dilution Ratio	WB: 1:500 ;IHC-P: 1:50-2:00 Western blot analysis of extracts from A431 (lane 2(20μg) and A431(lane 3(40μg) using Phospho-Met (HY-P80512) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Tyr1349 of Human Met.The exact sequence is proprietary to MCE.
Database	Entrez Gene: 4233 Human SwissProt: P08581 Human OMIM: 114550 Human
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (190 KB) User Guide for Antibodies (1077 KB)

Phospho-Met Antibody (YA175) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Met Antibody (YA175)

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