Phospho-IRF3 (Ser386) Antibody

Cat. No.: HY-P81022: Data Sheet
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-IRF3 (Ser386) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-IRF3 (Ser386).

For research use only. We do not sell to patients.

Size	Price	Stock
10 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time

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Phospho-IRF3 (Ser386) Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-IRF3 (Ser386) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-IRF3 (Ser386).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q14653

Gene ID

3661 [NCBI]

Immunogen

Synthetic peptide corresponding to human Phospho-IRF3 (S386).The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Phosphorylated	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7(lane 2(20ug) and MCF-7(lane 3(40ug) using Phospho-IRF3 Antibody (HY-P81022) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

RRID

AB_3103027

Database

Entrez Gene: 3661 Human

SwissProt: Q14653 Human

OMIM: 616532 Human

Documentation

Data Sheet (235 KB) SDS (251 KB) User Guide for Antibodies (1077 KB)

Phospho-IRF3 (Ser386) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-IRF3 (Ser386) Antibody

Phospho-IRF3 (Ser386) Antibody Featured Recommendations:

Product Detail

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Background

Documentation

Phospho-IRF3 (Ser386) Antibody Related Classifications

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