Phospho-HSF1 (Ser326) Antibody(YA894)

Phospho-HSF1 (Ser326) Antibody(YA894) is an unconjugated, approximately 57 kDa, rabbit-derived, anti-HSF1 (Ser326)(YA894) monoclonal antibody. Phospho-HSF1 (Ser326) Antibody(YA894) can be used for: WB, IF-Cell, IHC-P, IP, FC expriments in human background without labeling.

Q00613

Predicted band size: 57 kDa；Observed band size: 80 kDa

Affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3103042

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Phospho-HSF1 Antibody (HY-P81050) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phospho-peptide corresponding to residues surrounding Ser326 of Human HSF1 aa 301-350 / 529.