Phospho-HSF1 (Ser326) Antibody(YA894)

Phospho-HSF1 (Ser326) Antibody(YA894) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-HSF1 (Ser326).

HSF1, a stress-responsive and DNA-binding transcription factor, is a central orchestrator of the heat shock response (HSR), leading to the transcriptional activation of a multitude of heat shock proteins (HSPs) crucial for cellular protection against damage. In unstressed conditions, HSF1 resides in a HSP90-containing multichaperone complex, maintaining an inactivated monomeric state. Upon exposure to stress, particularly heat, HSF1 undergoes homotrimerization and activates the transcription of HSP genes by binding to heat shock elements (HSEs) in their promoters. This transition is reversible, and during the recovery phase of the HSR, HSF1 returns to its unactivated form. Beyond transcriptional activation, HSF1 participates in various cellular processes, including chromatin-associated complex formation with TTC5/STRAP and p300/EP300, repression of Ras-induced c-fos gene transcription, regulation of pre-mRNA processing, nuclear export of stress-induced mRNA, and modulation of mitotic progression. Additionally, in the context of microbial infection, HSF1 plays a role in the reactivation of latent human immunodeficiency virus (HIV-1) transcription by binding to the HIV-1 long terminal repeat promoter.

Q00613

Predicted band size: 57 kDa；Observed band size: 80 kDa

Affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3103042

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000-1:2000; ICC: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Phospho-HSF1 Antibody (HY-P81050) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling Phospho-HSF1 (Ser326) with Phospho-HSF1 (Ser326) Antibody (HY-P81050) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-HSF1 (Ser326)Antibody (HY-P81050) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-HSF1 (Ser326) with Phospho-HSF1 (Ser326) Antibody (HY-P81050) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-HSF1 (Ser326)Antibody (HY-P81050)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phospho-peptide corresponding to residues surrounding Ser326 of Human HSF1 aa 301-350 / 529.