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  4. phospho-HSF1 (Ser326) Antibody (YA6363)

phospho-HSF1 (Ser326) Antibody (YA6363)

Cat. No.: HY-P86671
User Guide for Antibodies Technical Support

phospho-HSF1 (Ser326) Antibody (YA6363) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated HSF1 (Ser326). It can be applicated for WB, IHC-P, IHC-F, IF-Tissue, FC assays, in the background of human, mouse.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

phospho-HSF1 (Ser326) Antibody (YA6363) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated HSF1 (Ser326). It can be applicated for WB, IHC-P, IHC-F, IF-Tissue, FC assays, in the background of human, mouse.

Background

HSF1, a stress-responsive and DNA-binding transcription factor, is a central orchestrator of the heat shock response (HSR), leading to the transcriptional activation of a multitude of heat shock proteins (HSPs) crucial for cellular protection against damage. In unstressed conditions, HSF1 resides in a HSP90-containing multichaperone complex, maintaining an inactivated monomeric state. Upon exposure to stress, particularly heat, HSF1 undergoes homotrimerization and activates the transcription of HSP genes by binding to heat shock elements (HSEs) in their promoters. This transition is reversible, and during the recovery phase of the HSR, HSF1 returns to its unactivated form. Beyond transcriptional activation, HSF1 participates in various cellular processes, including chromatin-associated complex formation with TTC5/STRAP and p300/EP300, repression of Ras-induced c-fos gene transcription, regulation of pre-mRNA processing, nuclear export of stress-induced mRNA, and modulation of mitotic progression. Additionally, in the context of microbial infection, HSF1 plays a role in the reactivation of latent human immunodeficiency virus (HIV-1) transcription by binding to the HIV-1 long terminal repeat promoter.

Gene ID
SwissProt ID
Synonyms
HSF1(S326); HSF1(phospho S326); p-HSF1(phospho S326); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1; HSF1_HUMAN.
Molecular Weight

Predicted band size: 57 kDa

Purity

affinity purified.

Subcellular Location

Cytoplasm. Nucleus. Note=Cytoplasmic duringnormal growth. On activation, translocates to nuclear stressgranules. Colocalizes with SUMO1 in nuclear stress granules.

Conjugation

Non-conjugated

Modification

Phosphorylated

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Monoclonal

Host

Rabbit

Reactivity

Human, Mouse

Predicted Reactivity

Rat

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
Dilution Ratio

WB: 1:500-2000; IHC-P: 1:50-200; IHC-F: 1:50-200; IF: 1:50-200; Flow-Cyt: 2ug/Test

Application

WB, IHC-P, IHC-F, IF-Tissue, FC

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326 aa L(p-S)PT

Documentation

phospho-HSF1 (Ser326) Antibody (YA6363) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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phospho-HSF1 (Ser326) Antibody (YA6363)
Cat. No.:
HY-P86671
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