Phospho-Creb (Ser133) Antibody (YA210)

Phospho-Creb (Ser133) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 35 kDa, targeting to Phospho-Creb (S133). It can be used for WB,ICC,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.

Free

P16220

Predicted band size: 35 kDa; Observed band size: 42 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Phosphorylated

AB_3102531

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1,000 ;ICC: 1:50-1:100 ;IHC-P: 1:50-1:400 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) using Phospho-Creb(HY-P80457 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of C6 cells labeling Phospho-Creb (Ser133) with Phospho-Creb (Ser133) Antibody (HY-P80457) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Creb (Ser133) Antibody (HY-P80457) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-Creb (Ser133) with Phospho-Creb (Ser133) Antibody (HY-P80457) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Creb (Ser133) Antibody (HY-P80457)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser133 of Human Creb.The exact sequence is proprietary to MCE.