Phospho-c-Jun(Ser63) Antibody (YA214)

Phospho-c-Jun(Ser63) Antibody (YA214) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-c-Jun(Ser63).

c-Jun is a transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. It heterodimerizes with FOS family proteins to form the AP-1 transcription complex, enhancing both DNA binding and transcriptional activity (by similarity). Together with FOSB, it participates in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L and inducing its transcription upon TCR/CD3 signaling pathway activation. When phosphorylated by HIPK3, c-Jun promotes NR5A1 activity, boosting steroidogenic gene expression in response to cAMP pathway stimulation. In colorectal cancer (CRC) cells, it mediates KRAS-activated transcriptional upregulation of USP28 and directly binds to the USP28 promoter. During Epstein-Barr virus (EBV) infection, c-Jun binds to the viral BZLF1 Z promoter to activate BZLF1 expression.

Free

P05412

Predicted band size: 36 kDa； Observed band size: 41 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Phosphorylated

AB_3102530

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:2000; ICC: 1:50-1:200; IHC-P: 1:50-1:500

• 
  Western blot analysis of extracts from C6 (lane 2(20μg) , NIH3T3 (lane 3(20μg) and HEK293(lane 4(20μg) using Phospho-c-Jun(Ser63) (HY-P80456) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Phospho-c-Jun(Ser63) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80456, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Phospho-c-Jun(Ser63) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80456, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser63 of Human c-Jun.AA range:31-80.