Phospho-ATM (Ser1981) Antibody (YA3181)

Phospho-ATM (Ser1981) Antibody (YA3181) is a non-conjugated IgG antibody, targeting Phospho-ATM (Ser1981), with a predicted molecular weight of 351 kDa. Phospho-ATM (Ser1981) Antibody (YA3181) can be used for WB, IHC-P, ICC/IF, IP experiment in human background.

ATM is a serine/threonine protein kinase that activates checkpoint signaling in response to double-strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing radiation, functioning as a DNA damage sensor. It recognizes the substrate consensus sequence [ST]-Q and phosphorylates histone variant H2AX at Ser-139 at DSBs, thereby regulating the DNA damage response mechanism (By similarity). Additionally, ATM plays a role in pre-B cell allelic exclusion by repositioning immunoglobulin heavy chain alleles to enforce clonality. It is involved in signal transduction, cell cycle control, and may act as a tumor suppressor. ATM phosphorylates various substrates, including DYRK2, CHEK2, p53/TP53, BRCA1, etc., modulating their functions. Under genotoxic stress, ATM prevents ubiquitin-mediated degradation of DYRK2 and enhances the DNA damage response by phosphorylating ATF2 and ERCC6. Furthermore, ATM participates in pexophagy by phosphorylating PEX5, promoting its ubiquitination and degradation.

Q13315

Predicted band size: 351 kDa; Observed band size: 370 kDa

Affinity Chromatography

Non-conjugated

Phosphorylated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/50

WB, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Phospho-ATM (S1981)