PARP1 Antibody (YA246)

PARP1 Antibody (YA246) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PARP1.

Cleaved PARP is a poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. It catalyzes ADP-ribosylation of glutamate, aspartate, serine, histidine, or tyrosine residues, with serine ADP-ribosylation being the primary form in response to DNA damage. Specificity for different amino acids is conferred by interacting factors such as HPF1 and NMNAT1. In DNA break repair, Cleaved PARP recognizes and binds DNA breaks in chromatin, recruits HPF1, and promotes serine ADP-ribosylation of histones (e.g., H2BS6ADPr and H3S10ADPr), facilitating chromatin decompaction and repair factor recruitment. It also participates in double-strand break repair, transcription regulation, programmed cell death, membrane repair, adipogenesis, and innate immunity. During apoptosis, Cleaved PARP is cleaved by caspase-3 and caspase-7, translocates to the cytoplasm, and induces AIFM1-mediated apoptosis through auto-poly-ADP-ribosylation. This cleaved form irreversibly binds DNA breaks, interfering with DNA repair and promoting DNA damage-induced apoptosis.

Free

P09874

Predicted band size: 113 kDa; Observed band size: 113 kDa

Protein A affinity purified.

Nucleus, nucleolus, chromosome.

Non-conjugated

Unmodified

AB_3102388

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:500 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)using PARP1 (HY-P80263) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80263, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling PARP1with PARP1antibody (HY-P80263) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PARP1 antibody (HY-P80263) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80263, 1/4000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80263, 1/4000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within N-terminal human PARP1.