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  5. Pan-Cadherin Antibody (YA247)

Pan-Cadherin Antibody (YA247)

Cat. No.: HY-P80262
COA
SDS
User Guide for Antibodies Technical Support

Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.
Background

CDH2 is a calcium-dependent cell adhesion protein that preferentially mediates homotypic cell-cell adhesion through dimerization with CDH2 chains from other cells, thereby contributing to the sorting of heterogeneous cell types. In the adult subependymal zone, CDH2 regulates neural stem cell quiescence by mediating their anchorage to ependymocytes; cleavage by MMP24 disrupts this anchorage, modulating quiescence. Additionally, CDH2 plays a role in forming cell-to-cell junctions between pancreatic beta cells and neural crest stem (NCS) cells, promoting process formation in NCS cells (By similarity). It is essential for proper neurite branching (By similarity) and pre- and postsynaptic organization (By similarity). CDH2 may participate in neuronal recognition mechanisms and potentially regulates dendritic spine density in hippocampal neurons.
Tag

Free
Gene ID
SwissProt ID
Synonyms
Cadherin antibody; CDH3 antibody; CDHP antibody; 7B4 antigen antibody; Cadherin-1 antibody; Cadherin-2 antibody; Cadherin-3 antibody; Cadherin-4 antibody; Cadherin-5 antibody; CAM 120/80 antibody; CD144 antibody; CD324 antibody; CD325 antibody; Cdh4 antibody; CDH5 antibody; CDHE antibody; CDHN antibody; E-cadherin antibody; Epithelial cadherin antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; P-cadherin antibody; Placental cadherin antibody; UVO antibody; Uvomorulin antibody
Molecular Weight

Predicted band size: 100 kDa; Observed band size: 130 kDa
Purity

Protein A affinity purified.
Subcellular Location

Cell membrane, Cell junction, Endosome, Golgi apparatus.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Tags & Cell Markers
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:1000; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

  • Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application

WB, ICC/IF, IHC-P, IP, FC
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide within C-terminal human CDH2.
Database

Entrez Gene: 1001 Human ; 1002 Human ; 1003 Human ; 1004 Human ; 1005 Human ; 1006 Human ; 1008 Human ; 1009 Human ; 1012 Human ; 1014 Human ; 1015 Human ; 999 Human ;

SwissProt: P12830 Human ; P19022 Human ; P22223 Human ; P33151 Human ; P55283 Human ; P39038 Mouse ;

OMIM: 137215 Human

Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Pan-Cadherin Antibody (YA247) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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