PAIP1 Antibody (YA1528)

PAIP1 Antibody (YA1528) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1528), targeting PAIP1, with a predicted molecular weight of 54 kDa (observed band size: 54 kDa). PAIP1 Antibody (YA1528) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.

PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1, and it competes with PAIP2 for binding to PABPC1. The association of PAIP1 with EIF4A and PABPC1 may potentiate contacts between mRNA termini. It may also be involved in translationally coupled mRNA turnover. PAIP1 is implicated, along with other RNA-binding proteins, in the cytoplasmic deadenylation/translational and decay interplay of FOS mRNA, mediated by the major coding-region determinant of instability (mCRD) domain. by similar: During microbial infection, PAIP1 interacts with the SARS coronavirus SARS-CoV NSP3 protein and plays an important role in viral protein synthesis.

Q9H074

Predicted band size: 54 kDa; Observed band size: 54 kDa

Affinity Purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

• 
  Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), Hela (lane 2, 20 μg), and A549 (lane 3, 20 μg) using PAIP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human PAIP1