p53 (acetyl K370)Antibody (YA248)

p53 (acetyl K370)Antibody (YA248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p53 (acetyl K370).

p53 protein functions as a tumor suppressor in numerous tumor types, exerting its effects through the induction of growth arrest or apoptosis depending on the physiological conditions and cell type. In the realm of cell cycle regulation, p53 acts as a trans-activator that negatively regulates cell division by overseeing a set of genes crucial for this process, including an inhibitor of cyclin-dependent kinases. Apoptosis induction by p53 is thought to occur through the stimulation of BAX and FAS antigen expression or repression of Bcl-2 expression. The multifaceted role of p53 underscores its significance in orchestrating cellular responses to maintain genomic integrity and counteract aberrant cell proliferation.

Free

P04637

Predicted band size: 44 kDa; Observed band size: 53 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.

Non-conjugated

Acetylated

AB_3102387

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000 ;ICC/IF: 1:50 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from HepG2 (lane 2(20μg) or lane 3(40μg)), using P53(HY-P80260) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80260, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human p53.AA range:343-392.