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  5. Phospho-RIP(S166) Antibody (YA1284)

Phospho-RIP(S166) Antibody (YA1284)

Cat. No.: HY-P81539
COA
SDS
User Guide for Antibodies Technical Support

Phospho-RIP(S166) Antibody (YA1284) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1284), targeting p-RIPK1(S166), with a predicted molecular weight of 75 kDa, (observed band size: 75 kDa). Phospho-RIP(S166) Antibody (YA1284) can be used for WB, IHC-F, IHC-P, FC, ICC/IF, IP experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

Publications Citing Use of MCE Phospho-RIP(S166) Antibody (YA1284)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Phospho-RIP(S166) Antibody (YA1284) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1284), targeting p-RIPK1(S166), with a predicted molecular weight of 75 kDa, (observed band size: 75 kDa). Phospho-RIP(S166) Antibody (YA1284) can be used for WB, IHC-F, IHC-P, FC, ICC/IF, IP experiment in human, mouse, rat background.
Background

p-RIPK1 is a serine-threonine kinase that serves as a key regulator of TNF-mediated apoptosis, necroptosis, and inflammatory pathways. It exhibits dual kinase-dependent and -independent functions: its kinase activity governs cell death (e.g., by forming complex IIa for apoptosis and complex IIb for necroptosis), while its scaffold role promotes cell survival via recruitment to the TNF-R1 signaling complex (TNF-RSC/complex I) and activation of the canonical NF-kappa-B pathway. Under physiological conditions, p-RIPK1 inhibits RIPK3-dependent necroptosis (through FADD-mediated CASP8 recruitment to cleave RIPK1) and aberrant apoptosis during embryogenesis (by blocking TRADD-FADD interaction). It also drives inflammatory responses by enhancing pro-inflammatory cytokine (e.g., IL6) production. By similarity, it reciprocally phosphorylates RIPK3 and phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner to activate the MAP3K5-JNK apoptotic cascade.
Gene ID
SwissProt ID
Synonyms
Cell death protein RIP,Receptor-interacting protein 1
Molecular Weight

Predicted band size: 75 kDa,Observed band size:75 kDa
Purity

ProA affinity purified IgG
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Mouse
Dilution Ratio

WB: 1/1000-1/2000; IP: 1/50-1/500; ICC/IF: 1/100-1/500; IHC-P: 1/100-1/500; IHC-F: 1/100-1/500
Application

WB, IHC-P, IHC-F, ICC/IF, IP
Appearance

Liquid
Formulation

Supplied in PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic phospho-peptide corresponding to residues surrounding serine 166 of Mouse RIPK1
Database

Entrez Gene: 8737 Human ; 19766 Mouse ; 306886 Rat

SwissProt: Q13546 Human ; Q60855 Mouse ;

OMIM: 618108 Human

Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-RIP(S166) Antibody (YA1284) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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