NQO1 Antibody (YA261)

NQO1 Antibody (YA261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NQO1.

NQO1 is a flavin-containing quinone reductase that catalyzes the two-electron reduction of quinones to hydroquinones using NADH or NADPH as electron donors. It operates via a ping-pong kinetic mechanism, sequentially transferring electrons from NAD(P)H to the flavin cofactor and then to the quinone substrate, thereby bypassing the formation of semiquinone intermediates and reactive oxygen species (By similarity). Its primary role involves regulating cellular redox homeostasis through quinone detoxification, reducing components of the plasma membrane redox system (e.g., coenzyme Q and vitamin quinones) to generate antioxidant hydroquinone forms, while potentially scavenging superoxide to prevent hydroquinone oxidation and facilitate excretion. Paradoxically, it can also activate certain quinones by generating redox-reactive hydroquinones with DNA cross-linking antitumor properties. Additionally, NQO1 functions as a gatekeeper of the 20S proteasome core; under oxidative stress, it interacts with tumor suppressors TP53 and TP73 in an NADH-dependent manner to inhibit their ubiquitin-independent degradation by the 20S proteasome.

Free

P15559

Predicted band size: 31 kDa; Observed band size: 31 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102381

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:5000; ICC/IF: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human NQO1.AA range:1-49.