NQO1 Antibody (YA6218)

NQO1 Antibody (YA6218) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NQO1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

NQO1 is a flavin-containing quinone reductase that catalyzes the two-electron reduction of quinones to hydroquinones using NADH or NADPH as electron donors. It operates via a ping-pong kinetic mechanism, sequentially transferring electrons from NAD(P)H to the flavin cofactor and then to the quinone substrate, thereby bypassing the formation of semiquinone intermediates and reactive oxygen species (By similarity). Its primary role involves regulating cellular redox homeostasis through quinone detoxification, reducing components of the plasma membrane redox system (e.g., coenzyme Q and vitamin quinones) to generate antioxidant hydroquinone forms, while potentially scavenging superoxide to prevent hydroquinone oxidation and facilitate excretion. Paradoxically, it can also activate certain quinones by generating redox-reactive hydroquinones with DNA cross-linking antitumor properties. Additionally, NQO1 functions as a gatekeeper of the 20S proteasome core; under oxidative stress, it interacts with tumor suppressors TP53 and TP73 in an NADH-dependent manner to inhibit their ubiquitin-independent degradation by the 20S proteasome.

P15559

Predicted band size: 31 kDa; Observed band size: 31 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:1000-1:5000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200,

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG