NMDAR2A Antibody (YA264)

NMDAR2A Antibody (YA264) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NMDAR2A.

GRIN2A is a component of N-methyl-D-aspartate (NMDA) receptors, which function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg²⁺. These receptors contribute to synaptic plasticity for learning and memory formation by participating in the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similar). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine to the GluN1 subunit, along with membrane depolarization to relieve Mg²⁺ inhibition. NMDA receptors mediate simultaneous potassium efflux and influx of calcium and sodium (By similar). Each GluN2 subunit confers distinct channel properties, including activation, deactivation, and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators. GRIN2A participates in synaptic plasticity regulation through activation by L-glutamate released by BEST1 into the synaptic cleft upon F2R/PAR-1 activation in astrocytes (By similar).

Free

Q12879

Predicted band size: 165 kDa; Observed band size: 165 kDa

Protein A affinity purified.

Cell membrane, postsynaptic cell membrane, dendritic spine, synapse, Cytoplasmic vesicle membrane.

Non-conjugated

Unmodified

AB_3102867

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500; IHC-P: 1:50-1:100; IF: 1:200

• 
  Immunocytochemistry analysis of Hela cells labeling NMDAR2A with NMDAR2A Antibody (HY-P80248)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NMDAR2A Antibody (HY-P80248) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of U251 cells labeling NMDAR2A with NMDAR2A Antibody (HY-P80248) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NMDAR2A Antibody (HY-P80248) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using NMDAR2A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80248, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using NMDAR2A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80248, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human NMDAR2A.AA range:1356-1405.