Myosin light chain kinase/MLCK Antibody (YA3133)

Cat. No.: HY-P83388: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Myosin light chain kinase/MLCK Antibody (YA3133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin light chain kinase/MLCK.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Myosin light chain kinase/MLCK Antibody (YA3133) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Myosin light chain kinase/MLCK Antibody (YA3133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin light chain kinase/MLCK.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 211 kDa;

Observed band size: 130-140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15746

Gene ID

85366 [NCBI]

Immunogen

A synthesized peptide derived from human Myosin light chain kinase aa1850-1914.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of NIH-3T3 cells labeling MYLK with MYLK antibody (HY-P83388) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MYLK antibody (HY-P83388) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling MYLK with MYLK antibody (HY-P83388) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MYLK antibody (HY-P83388) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3105501

Database

Entrez Gene: 85366 Human ; 228785 Mouse ; 117558 Rat

SwissProt: Q15746 Human ; Q8VCR8 Mouse ; P20689 Rat

OMIM: 613780 Human

Research Field

Signal Transduction

Synonyms

KMLS; KRP; MLCK; MYLK; myosin light chain kinase; smooth muscle and non-muscle isozymes; myosin light polypeptide kinase; telokin

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Myosin light chain kinase/MLCK Antibody (YA3133) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Myosin light chain kinase/MLCK Antibody (YA3133)

Myosin light chain kinase/MLCK Antibody (YA3133) Featured Recommendations:

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Myosin light chain kinase/MLCK Antibody (YA3133) Related Classifications

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