mTOR Antibody (YA6081)

mTOR Antibody (YA6081) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to mTOR. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

mTOR is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to hormones, growth factors, nutrients, energy, and stress signals. It functions through two structurally and functionally distinct complexes, mTORC1 and mTORC2. mTORC1 is recruited to the lysosome membrane upon nutrient or growth factor stimulation, promoting protein, lipid, and nucleotide synthesis by phosphorylating key regulators of translation (e.g., EIF4EBP1, RPS6KB1/2) while suppressing autophagy (e.g., via ULK1, DAP, AMBRA1 phosphorylation). mTORC2 responds to growth factors by phosphorylating AGC kinase family members (e.g., AKT, PKC, SGK1) to regulate proliferation, cytoskeletal organization, and lipogenesis. Additionally, mTOR modulates mitochondrial biogenesis (by similar), insulin signaling (by similar), circadian rhythms (by similar), and pyroptosis (e.g., GSDMD oligomerization).

P42345

Predicted band size: 289 kDa; Observed band size: 260 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG