MPO Antibody(YA3392)

MPO Antibody is an unconjugated, approximately 84 kDa, mouse-derived, anti-MPO monoclonal antibody. MPO Antibody can be used for: WB, IHC-P, IF-Cell, IF-Tissue expriments in human, mouse, rat background without labeling.

MPO is part of the host defense system of polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. In stimulated PMNs, MPO catalyzes the production of hypohalous acids (primarily hypochlorous acid under physiological conditions) and other toxic intermediates, greatly enhancing PMN microbicidal activity. Additionally, MPO mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage.

P05164

Predicted band size: 84 kDa; Observed band size: 80-90 kDa

affinity purified

Non-conjugated

Unmodified

AB_3105041

Primary Antibody; Recombinant Mouse Monoclonal Antibody

Recombinant, Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:1,000;IHC-P: 1:5,000-1:10,000;IF-Cell: 1:100;IF-Tissue: 1:1,000-1:2,000;

• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using MPO Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using MPO Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within human Myeloperoxidase aa 550-745.