MIF Antibody (YA1912)

Cat. No.: HY-P82167: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

MIF Antibody (YA1912) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MIF.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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MIF Antibody (YA1912) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

MIF Antibody (YA1912) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MIF.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 12 kDa;

Observed band size: 13 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P14174

Gene ID

4282 [NCBI]

Immunogen

A synthesized peptide derived from human MIF aa60-115/115.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat(lane 2(20ug) , THP-1(lane 3(20ug) and HL-60(lane 4(20ug)using MIF Antibody (HY-P82167) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

RRID

AB_3104280

Database

Entrez Gene: 4282 Human ; 17319 Mouse ; 81683 Rat

SwissProt: P14174 Human ; P34884 Mouse ; P30904 Rat

OMIM: 604302 Human

Research Field

Immunology

Synonyms

MIF; GLIF; MMIF; Macrophage migration inhibitory factor; MIF; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

MIF Antibody (YA1912) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MIF Antibody (YA1912)

MIF Antibody (YA1912) Featured Recommendations:

Product Detail

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Background

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MIF Antibody (YA1912) Related Classifications

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